ligand blotting

ligand blotting
Вестерн-блоттинг, белковый б. — техника для выявления белков, аналогичная Саузерн-блоттингу. Белки разделяются при электрофорезе в полиакриламидном геле, переносятся на мембрану и затем метятся с помощью радиоактивной или флуоресцентной метки или с помощью антител (иммунопробы).

Англо-русский толковый словарь генетических терминов. — М.: Изд-во ВНИРО. . 1995.

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  • T-cadherin — also known as cadherin 13, H cadherin (heart) (CDH13) is a unique member of cadherin superfamily because it lacks the transmembrane and cytoplasmic domains and is anchored to the cells membrane through the GPI anchor. Classical cadherins are… …   Wikipedia

  • 11β-hydroxysteroid dehydrogenase type 1 — is an NADPH dependent enzyme highly expressed in key metabolic tissues including liver, adipose tissue, and the central nervous system. In these tissues, HSD11B1 reduces cortisone to the active hormone cortisol that activates glucocorticoid… …   Wikipedia

  • HDLBP — High density lipoprotein binding protein (vigilin), also known as HDLBP, is a human gene.cite web | title = Entrez Gene: HDLBP high density lipoprotein binding protein (vigilin)| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene… …   Wikipedia

  • Northern blot — The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample.[1][2] …   Wikipedia

  • Southern-Blot — Autoradiogramm eines Southern Blots Beim Southern Blot handelt es sich um eine 1975 von Edwin Southern entwickelte molekularbiologische Untersuchungsmethode für DNA.[1] Sie ermöglicht den Nachweis einer Gensequenz in einem komplexen DNA–Gemisch… …   Deutsch Wikipedia

  • Southern Blot — Autoradiogramm eines Southern Blots Beim Southern Blot handelt es sich um eine 1975 von Edwin Southern entwickelte molekularbiologische Untersuchungsmethode für die DNA.[1] Sie ermöglicht den Nachweis einer Gensequenz in einem komplexen DNA… …   Deutsch Wikipedia

  • Gel electrophoresis — apparatus – An agarose gel is placed in this buffer filled box and electrical field is applied via the power supply to the rear. The negative terminal is at the far end (black wire), so DNA migrates toward the camera. Classification… …   Wikipedia

  • Far-Eastern blot — Far Eastern blotting is a technique developed in the 1990s by T. Taki and colleaguesCitation | author = D. Ishikawa and T. Taki title = Micro scale analysis of lipids by far eastern blot (TLC blot) journal = Nihon yukagaku kaishi volume = 47… …   Wikipedia

  • Protein purification — is a series of processes intended to isolate a single type of protein from a complex mixture. Protein purification is vital for the characterisation of the function, structure and interactions of the protein of interest. The starting material is… …   Wikipedia

  • Lipid raft — organisation, region (1) is standard lipid bilayer, while region (2) is a lipid raft. The plasma membrane of cells is made of a combination of glycosphingolipids and protein receptors organized in glycolipoprotein microdomains termed lipid… …   Wikipedia

  • Methods to investigate protein–protein interactions — There are many methods to investigate protein–protein interactions. Each of the approaches has its own strengths and weaknesses, especially with regard to the sensitivity and specificity of the method. A high sensitivity means that many of the… …   Wikipedia


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